Bordet-Gengou Medium (ATCC Medium 35)

[所属分类:培养基配方] [发布时间:2020-1-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bordet-Gengou Medium
(ATCC Medium 35)

培养基配方
Composition per liter:
Agar .........................................................20.0g
Glycerol .....................................................10.0g
Proteose peptone..............................................10.0g
NaCl...........................................................5.5g
Pancreatic digest of casein....................................5.0g
Peptic digest of animal tissue.................................5.0g
Potato, solids from infusion...................................4.5g
Rabbit blood................................................150.0mL
pH 6.7± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium: Add 10.0g of glycerol to 980.0mL of dis-
tilled/deionized water. Add other components, except rabbit blood, to
the glycerol solution. Mix thoroughly. Heat with occasional agitation
of the medium. Boil for 1 min. Autoclave for 15 min at 15 psi pressure–
121°C. Cool medium to 50°C. Aseptically add 150.0mL of rabbit
blood (prewarmed to 35°C). Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes. Allow tubes to cool in a slanted
position.
Use: For the detection and isolation of Bordetella pertussis and Bor-
detella parapertussis from clinical specimens. The medium is rendered
selective by the addition of methicillin. Bordetella pertussis appears as
small (<1mm), smooth, pearl-like colonies surrounded by a narrow
zone of hemolysis. Bordetella parapertussis appears as brown, non-
shiny colonies with a green-black coloration on the reverse side. Bor-
detella bronchiseptica appears as brown, nonshiny, moderately
sized colonies with a roughly pitted surface.