Blood Agar Base, Sheep

[所属分类:培养基配方] [发布时间:2019-12-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar Base, Sheep

培养基配方
Composition per liter:
Pancreatic digest of casein...................................14.0g
Agar..........................................................12.5g
NaCl...........................................................5.0g
Peptone .......................................................4.5g
Yeast extract..................................................4.5g
Sheep blood, defibrinated....................................70.0mL
ph 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation : Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool the basal medium to 45°–50°C. Aseptically add
70.0mL of sterile, defibrinated sheep blood. Pour into sterile Petri dish-
es.
Use:  For giving improved hemolytic reactions with sheep blood.