Blood Agar Base (ATCC Medium 368)

[所属分类:培养基配方] [发布时间:2019-12-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar Base
(ATCC Medium 368)

培养基配方
Composition per liter:
Beef heart, infusion from....................................500.0g
Agar..........................................................15.0g
Tryptose......................................................10.0g
NaCl...........................................................5.0g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat with frequent
agitation and boil for 1 min to completely dissolve. Autoclave for 15
min at 15 psi pressure–121°C. Cool the basal medium to 45°–50°C.
Aseptically add sterile, defibrinated blood to a final concentration of
5%. Mix thoroughly and pour into sterile Petri dishes.
Use:  For the isolation, cultivation, and detection of hemolytic activity
of staphylococci, streptococci, and other fastidious microorganisms.