BLE HiVeg Broth Base with Listeria Selective Supplement (Buffered Listeria Enrichment HiVeg Broth Base with Listeria Selective Supplement)

[所属分类:培养基配方] [发布时间:2019-12-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BLE HiVeg Broth Base
with Listeria Selective Supplement
(Buffered Listeria Enrichment HiVeg Broth Base
with Listeria Selective Supplement)

培养基配方
Composition per liter:
Plant hydrolysate .......................................17.0g
Na2HPO4 , anhydrous.......................................9.6g
Yeast extract.............................................6.0g
NaCl......................................................5.0g
Papaic digest of soybean meal.............................3.0g
KH2PO4 ...................................................2.5g
Glucose ..................................................2.5g
Sodium pyruvate...........................................1.0g
Listeria selective supplement............................5.0mL
pH 7.3 ± 0.2 at 25°C
Listeria Selective Supplement:
Composition per 5.0mL:
Cycloheximide.................................................50.0mg
Nalidixic acid................................................40.0mg
Acriflavin hydrochloride......................................15.0mg
Preparation of Listeria Selective Supplement: Add compo-
nents to distilled/deionized water and bring volume to 5.0mL. Mix
thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Source: This medium, without Listeria selective supplement, is avail-
able as a premixed powder from HiMedia.
Preparation of Medium:  Add components, except Listeria selec-
tive supplement, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL
of sterile Listeria selective supplement. Mix thoroughly.
Use:  For the enrichment and isolation of Listeria monocytogenes.