Blastococcus aggregatus Medium

[所属分类:培养基配方] [发布时间:2019-12-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blastococcus aggregatus Medium

培养基配方
Composition per 1001.0mL:
Tryptone........................................................2.0g
Yeast extract...................................................2.0g
Tris(hydroxymethyl)amino
methane·HCl buffer.............................................1.0g
KNO3 ...........................................................0.5g
Sodium glycerophosphate.........................................0.1g
Artificial seawater.............................................1.0L
Trace elements solution .......................................1.0mL
pH 7.0 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
Commercially available marine aquarium salts mixture ........variable
Preparation of Artificial Seawater: Add commercially available
marine aquarium salts mixture. Prepare according to manufacturer’s
recommendations. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
H3BO3 .....................................................2.85g
MnCl2 ·4H2O................................................1.8g
Sodium tartrate............................................1.77g
FeSO4 .....................................................1.36g
CoCl2 ·6H2O..............................................40.4mg
CuCl2 ·2H2O..............................................26.9mg
Na2MoO4 ·2H2O............................................25.2mg
ZnCl2 ....................................................20.8mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium:  Combine components. Mix thoroughly.
Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the cultivation of Blastococcus aggregatus.