Blastobacter denitrificans Agar

[所属分类:培养基配方] [发布时间:2019-12-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blastobacter denitrificans Agar

培养基配方
Composition per liter:
Agar.........................................................15.0g
Pancreatic digest of casein...................................2.0g
Beef extract..................................................0.5g
Yeast extract.................................................0.5g
Sodium acetate................................................0.2g
Glucose solution............................................10.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose.....................................................2.5g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 10.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of
sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Blastobacter denitrificans.