Bismuth Sulfite Agar Wilson and Blair (BAM 19)

[所属分类:培养基配方] [发布时间:2019-12-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bismuth Sulfite Agar Wilson and Blair
(BAM 19)

培养基配方
Composition per liter:
Agar..........................................................20.0g
Pancreatic digest of casein...................................10.0g
Bi2(SO3)3 .....................................................8.0g
Beef extract...................................................5.0g
Glucose........................................................5.0g
Na2HPO4 .......................................................4.0g
FeSO4 ·7H2O...................................................0.3g
Brilliant Green..............................................0.025g
pH 7.7 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Boil for 1 min. Do not autoclave. Cool to 45°–
50°C. Pour into sterile Petri dishes while gently shaking flask to dis-
perse precipitate. Let plates dry for about 2h with lids partially re-
moved. Use plates the within one day of preparation; medium loses
selectivity after 48h.
Use: For the selective isolation and identification of Salmonella typhi
and other enteric bacilli. Salmonella typhi appears as flat, black, “rab-
bit-eye” colonies surrounded by a zone of black with a metallic sheen.