Bismuth Sulfite Agar, HiVeg

[所属分类:培养基配方] [发布时间:2019-12-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bismuth Sulfite Agar, HiVeg

培养基配方
Composition per liter:
Agar............................................................20.0g
Plant peptone ..................................................10.0g
Bismuth sulfite indicator........................................8.0g
Glucose..........................................................5.0g
Plant extract ...................................................5.0g
Na2HPO4 .........................................................4.0g
FeSO4 ...........................................................0.3g
Brilliant Green ...............................................0.025g
pH 7.7 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Boil for 1 min. Do not autoclave. Cool to 45°–
50°C. Mix thoroughly. The sensitivity of the medium depends largely
upon uniform dispersion of precipitated bismuth sulfite in the final gel
which should be dispersed before pouring the plates. Pour into sterile
Petri dishes while gently shaking flask to disperse precipitate. Use
plates the same day as prepared.
Use: For the selective isolation and identification of Salmonella typhi
and other enteric bacilli. Salmonella typhi appears as flat, black, “rab-
bit-eye” colonies surrounded by a zone of black with a metallic sheen.