BIN Medium

[所属分类:培养基配方] [发布时间:2019-12-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BIN Medium

培养基配方
Composition per liter:
Beef heart, infusion from...................................250.0g
Calf brains, infusion from..................................200.0g
Agar.........................................................15.0g
Proteose peptone.............................................10.0g
NaCl..........................................................5.0g
Na2HPO4 ......................................................2.5g
Glucose.......................................................2.0g
Irgasan solution.............................................4.0mL
Crystal Violet solution......................................1.0mL
Sodium cholate solution .....................................1.0mL
Sodium deoxycholate solution.................................1.0mL
Nystatin solution............................................1.0mL
pH 7.4 ± 0.2 at 25°C
Sodium Cholate Solution:
Composition per 100.0mL:
Sodium cholate................................................5.0g
Preparation of Sodium Cholate Solution: Add sodium cholate
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Gently heat while stirring and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Sodium Deoxycholate Solution:
Composition per 100.0mL:
Sodium deoxycholate..........................................5.0g
Preparation of Sodium Deoxycholate Solution: Add sodium
deoxycholate to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Gently heat while stirring and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool 25°C.
Irgasan Solution:
Composition per 50.0mL:
Irgasan DP300 ...............................................10.0mg
Ethanol, 90%.................................................50.0mL
Preparation of Irgasan Solution: Add irgasan to 90% ethanol and
bring volume to 50.0mL. Mix thoroughly.
Crystal Violet Solution:
Composition per 10.0mL:
Crystal Violet...............................................10.0mg
Preparation of Crystal Violet Solution: Add Crystal Violet to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Gently heat while stirring and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C.

Nystatin Solution:
Composition per 10.0mL:
Nystatin .....................................................2.5g
Preparation of Nystatin Solution: Add nystatin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except irgasan solu-
tion, Crystal Violet solution, sodium cholate solution, sodium deoxy-
cholate solution, and nystatin solution, to distilled/deionized water and
bring volume to 992.0mL. Mix thoroughly. Gently heat while stirring
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 85°C. Aseptially add 4.0mL irgasam solution. Mix thoroughly
to volatilize the ethanol. Cool to 50°C. Aseptially add 1.0mL each of
Crystal Violet solution, sodium cholate solution, sodium deoxycholate
solution, and nystatin solution. Mix thoroughly. Pour into sterile Petri
dishes.
Use:  For the efficient detection of Yersinia pestis from clinical and
other specimens. The formulation of this medium is based on brain
heart infusion agar, to which the selective agents irgasan, cholate salts,
Crystal Violet, and nystatin are introduced to enhance efficiency of
recovery of Y. pestis.