Bifidobacterium Medium

[所属分类:培养基配方] [发布时间:2019-12-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bifidobacterium Medium

培养基配方
Composition per liter:
Tryptic digest of casein......................................10.0g
Glucose ......................................................10.0g
Beef extract...................................................5.0g
Yeast extract..................................................5.0g
K2HPO4 ........................................................3.0g
Tween™ 80.....................................................1.0mL
Sodium ascorbate solution....................................25.0mL
L -Cysteine·HCl solution....................................25.0mL
pH 6.8 ± 0.2 at 25°C
Sodium Ascorbate Solution:
Composition per 25.0mL:
Sodium ascorbate..............................................10.0g
Preparation of Sodium Ascorbate Solution: Add sodium ascor-
bate to distilled/deionized water and bring volume to 25.0mL. Mix
thoroughly. Filter sterilize.
L -Cysteine·HCl Solution:
Composition per 25.0mL:
L -Cysteine·HCl...............................................0.5g
Preparation of  L -Cysteine·HCl Solution: Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 25.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except sodium ascor-
bate solution and  L -cysteine·HCl solution, to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Aseptically add 25.0mL of sterile sodium ascor-
bate solution and 25.0mL of sterile  L -cysteine·HCl solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks. Medium that
has not been freshly prepared should be heated in a steamer for 10 min
prior to the addition of ascorbate and  L -cysteine.
Use: For the cultivation and maintenance of Bifidobacterium adolescen-
tis, Bifidobacterium angulatum, Bifidobacterium animalis, Bifidobacte-
rium asteroides, Bifidobacterium bifidum, Bifidobacterium boum, Bifi-
dobacterium breve, Bifidobacterium catenulatum, Bifidobacterium
choerinum, Bifidobacterium coryneforme, Bifidobacterium cuniculi,
Bifidobacterium dentium, Bifidobacterium gallicum, Bifidobacterium
indicum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobac-
terium magnum, Bifidobacterium merycicum, Bifidobacterium mini-
mum, Bifidobacterium pseudocatenulatum, Bifidobacterium pseudo-
longum, Bifidobacterium pullorum, Bifidobacterium ruminantium,
Bifidobacterium saeculare, Bifidobacterium subtile, Bifidobacterium
suis, and Bifidobacterium thermophilum.