Benzoate Minimal Salts Medium

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Benzoate Minimal Salts Medium

培养基配方
Composition per liter:
K2HPO4 .......................................................10.0g
NaNH4 HPO4 ·4H2O..............................................3.5g
MgSO4 ·7H2O...................................................0.2g
Citric acid, anhydrous.........................................0.2g
Benzoate solution............................................25.0mL
pH 7.0 ± 0.2 at 25°C
Benzoate Solution:
Composition per 25.0mL:
Sodium benzoate................................................2.5g
Preparation of Benzoate Solution: Add sodium benzoate to dis-
tilled/deionized water and bring volume to 25.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 975.0mL. Mix thoroughly. Adjust pH to 7.0. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°C. Aseptically add
25.0mL of sterile benzoate solution. Mix thoroughly. Aseptically distrib-
ute into sterile tubes or flasks.
Use:  For the cultivation of microorganisms that can utilize benzoate as
a carbon source.