Beijerinck’s Thiobacillus Medium

[所属分类:培养基配方] [发布时间:2019-12-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beijerinck’s Thiobacillus Medium

培养基配方
Composition per liter:
Noble agar...................................................20.0g
Na2HPO4 ......................................................0.2g
MgCl2 ........................................................0.1g
NH4Cl ........................................................0.1g
Na2S2O3 solution ..........................................100.0mL
NaHCO3 solution.............................................10.0mL
pH 7.0–7.2 at 25°C
Na2S2O3 Solution:
Composition per 100.0mL:
Na2S2O3 .....................................................5.0g
Preparation of Na2S2O3 Solution: Add Na2S2O3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
NaHCO3 Solution:
Composition per 10.0mL:
NaHCO3 .....................................................1.0g
Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium:  Add components, except Na2S2O3 solu-
tion and NaHCO3 solution, to distilled/deionized water and bring vol-
ume to 890.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 100.0mL of sterile Na2S203 solution
and 10.0mL of sterile NaHCO3 solution. Mix thoroughly. Pour into
sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Thiobacillus thermophil-
ica.