Beijerinckia Agar

[所属分类:培养基配方] [发布时间:2019-12-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beijerinckia Agar

培养基配方
Composition per liter:
Agar.......................................................15.0g
K2HPO4 .....................................................0.8g
KH2PO4 .....................................................0.2g
MgSO4 ·7H2O................................................0.1g
FeSO4 ·7H2O..............................................20.0mg
Na2MoO4 ·2H2O.............................................5.0mg
ZnSO4 ·6H2O...............................................5.0mg
CuSO4 ·6H2O...............................................4.0mg
MnSO4 ·6H2O...............................................2.0mg
Glucose solution .........................................50.0mL
pH 6.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
D- Glucose................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 10.0mL of sterile glucose so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes
Use: For the cultivation and maintenance of Beijerinckia derxii, Bei-
jerinckia fluminensis, Beijerinckia indica, Beijerinckia mobilis, Beijer-
inckia species, and Clostridium barkeri.