Beef Extract Peptone Serum Medium

[所属分类:培养基配方] [发布时间:2019-12-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beef Extract Peptone Serum Medium

培养基配方
Composition per liter:
Agar..............................................................25.0g
Beef extract......................................................10.0g
Peptone ..........................................................10.0g
NaCl...............................................................1.0g
Bovine serum.....................................................50.0mL
pH 8.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 950.0mL. Mix thor-
oughly. Adjust pH to 8.5. Heat gently and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 50.0mL of sterile bovine serum. Pour into sterile Petri dishes or
leave in tubes.
Use:  For the cultivation and maintenance of Serratia marcescens.