Bdellovibrio Medium

[所属分类:培养基配方] [发布时间:2019-12-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bdellovibrio Medium

培养基配方
Composition per Petri dish:
Base layer agar...........................................10.0mL
Semisolid agar ...........................................10.0mL
Host medium...............................................1.0mL
Host Medium:
Composition per liter:
Yeast extract..............................................3.0g
Peptone ...................................................0.6g
Preparation of Host Medium: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15
psi pressure–121°C.
Base Layer Agar:
Composition per liter:
Agar......................................................19.0g
Yeast extract..............................................3.0g
Peptone ...................................................0.6g
Preparation of Base Layer Agar: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Adjust pH to 7.2. Distribute into tubes in
10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Semisolid Agar:
Composition per liter:
Agar..........................................................6.0g
Yeast extract.................................................3.0g
Peptone ......................................................0.6g
Preparation of Semisolid Agar: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Adjust pH to 7.2. Distribute into tubes in 10.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Inoculate appropriate bacterial host into
10.0mL of host medium. Hosts include Erwinia amylovora, Escheri-
chia coli, Serratia marcescens, or Pseudomonas putida. Incubate host
culture for 24–48 hr at 30°C. Melt the base layer agar and semisolid
agar. Pour the base layer agar into a sterile Petri dish. Allow base layer
agar to solidify. Cool the semisolid agar to 40°–45°C. Add 1.0mL of
the previously grown host culture. Mix thoroughly. Pour over the so-
lidified base layer agar.
Use: For the cultivation of Bdellovibrio bacteriovorus and Bdellovi-
brio starrii.