BCYE Selective Agar with GVPC (Buffered Charcoal Yeast Extract Selective Agar with Glycine, Vancomycin, Polymyxin B, and Cycloheximide)

[所属分类:培养基配方] [发布时间:2019-12-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCYE Selective Agar with GVPC
(Buffered Charcoal Yeast Extract Selective Agar with
Glycine, Vancomycin, Polymyxin B, and Cycloheximide)

培养基配方
Composition per 1014.0mL:
Agar..........................................................15.0g
Yeast extract.................................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)..................................10.0g
Charcoal, activated ..........................................2.0g
α-Ketoglutarate..............................................1.0g
Fe4(P2O7)3 ·9H2O............................................0.25g
Antibiotic solution.........................................10.0mL
L -Cysteine·HCl·H2O solution...............................4.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
L -Cysteine·HCl·H2O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O ........................................1.0g
Preparation of  L -Cysteine·HCl·H2O Solution: Add  L-
cysteine·HCl·H2O to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Antibiotic Solution:
Composition per 10.0mL:
Glycine........................................................3.0g
Cycloheximide.................................................0.08g
Vancomycin....................................................1.0mg
Polymyxin B.................................................79,200U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation  of  Medium:  Add  components,  except  L -
cysteine·HCl·H2O solution and antibiotic solution, to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Adjust medium
to pH 6.9 with 1N KOH. Heat gently and bring to boil for 1 min. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Add
4.0mL of  L- cysteine·HCl·H2O solution and 10.0mL of sterile antibiotic
solution. Mix thoroughly. Pour into sterile Petri dishes with constant
agitation to keep charcoal in suspension.
Use: For the isolation, cultivation, and maintenance of Legionella
pneumophila and other Legionella species from environmental and
clinical specimens. For the selective recovery of Legionella pneumo-
phila while reducing contaminating microorganisms from potable
water samples.