BCYE Selective Agar with CCVC (Buffered Charcoal Yeast Extract Selective Agar with Cephalothin, Colistin, Vancomycin, and Cycloheximide)

[所属分类:培养基配方] [发布时间:2019-12-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCYE Selective Agar with CCVC
(Buffered Charcoal Yeast Extract
Selective Agar with Cephalothin,
Colistin, Vancomycin, and Cycloheximide)

培养基配方
Composition per 1014.0mL:
Agar..........................................................15.0g
Yeast extract.................................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)...................................10.0g
Charcoal, activated ...........................................2.0g
α-Ketoglutarate...............................................1.0g
Fe4(P2O7)3 ·9H2O.............................................0.25g
Antibiotic solution...........................................10.0mL
Cysteine·HCl·H2O solution....................................4.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
L -Cysteine·HCl·H2O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O..........................................1.0g
Preparation of  L -Cysteine·HCl·H2O Solution: Add  L-
cysteine·HCl·H2O to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide............................................80.0mg
Colistin.................................................16.0mg
Cephalothin...............................................4.0mg
Vancomycin ...............................................0.5mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium:  Add components, except  L -cysteine and
antibiotic solutions, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Adjust medium to pH 6.9 with 1N KOH. Heat
gently and bring to boil for 1 min. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°–55°C. Add 4.0mL of  L- cysteine·HCl·H2O so-
lution and 10.0mL of sterile antibiotic solution. Mix thoroughly. Pour
into sterile Petri dishes with constant agitation to keep charcoal in sus-
pension.
Use:  For the isolation, cultivation, and maintenance of Legionella
pneumophila and other Legionella species from environmental and
clinical specimens. For the selective recovery of Legionella pneumo-
phila while reducing contaminating microorganisms from environ-
mental water samples.