Basic Mineral Medium

[所属分类:培养基配方] [发布时间:2019-12-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Basic Mineral Medium

培养基配方
Composition per liter:
NH4NO3 .....................................................2.5g
Na2HPO4 ·2H2O..............................................1.0g
MgSO4 ·7H2O................................................0.5g
Fe(SO4)3 ·5H2O............................................0.01g
Co(NO3)2 ·6H2O ..........................................0.005g
CaCl2 ·2H2O..............................................1.0mg
KH2PO4 ....................................................0.5mg
MnSO4 ·2H2O...............................................0.1mg
(NH4)6 Mo7O24 ·4H2O.......................................0.1mg
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: To supply the mineral nutrients necessary for the cultivation of a
wide variety of microorganisms. Various carbon sources can be added
as sterilized solutions for testing carbon utilization capabilities.