Base Cholesterol Medium

[所属分类:培养基配方] [发布时间:2019-12-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Base Cholesterol Medium

培养基配方
Composition per liter:
Casitone ...........................................10.0g
Yeast extract.......................................10.0g
Cholesterol, ash free ...............................2.0g
CaCl2 ...............................................1.0g
Lecithin, type IV....................................1.0g
Sodium thioglycolate.................................0.5g
Resazurin ..........................................1.0mg
Preparation of Medium: Prepare and dispense medium under
100% N2 . Add cholesterol and lecithin to distilled/deionized water and
bring volume to 200.0mL of water. Mix thoroughly. Sparge with 100%
N2  for 10 min. Add other components to distilled/deionized water and
bring volume to 800.0mL of water. Mix thoroughly. Combine the two
solutions. Adjust pH to 7.5 with KOH. Gently heat and bring to boiling.
Continue boiling while sparging with 100% N2 until the resazurin turns
colorless. Cool under 100% N2 . Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Mix well after autoclaving.
Use: For the cultivation of Eubacterium coprostanoligenes.