Basal Synthetic Medium

[所属分类:培养基配方] [发布时间:2019-12-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Basal Synthetic Medium

培养基配方
Composition per liter:
L- Glutamic acid...........................................20.0g
(NH4)2SO4 ..................................................4.0g
K2HPO4 ....................................................1.88g
KH2PO4 ....................................................0.57g
MgSO4 ·7H2O................................................0.2g
Salt solution ............................................10.0mL
Salt Solution:
Composition per liter:
FeCl3 ·6H2O................................................0.6g
MnCl2 ·4H2O................................................0.6g
ZnCl2 ......................................................0.6g
CuSO4 ·5H2O................................................0.6g
CaCl2 ·2H2O ...............................................0.6g
NaCl........................................................0.6g
Preparation of Salt Solution: Add components to 1.0L of dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use:  For the cultivation and maintenance of Acinetobacter lwoffii.