BAM SM Broth

[所属分类:培养基配方] [发布时间:2019-12-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BAM SM Broth

培养基配方
Composition per liter:
Yeast extract...............................................6.0g
Glucose ....................................................5.0g
KH2PO4 .....................................................3.0g
MgSO4 ·7H2O................................................0.5g
CaCl2 ·2H2O..............................................0.25g
(NH4)2SO4 ..................................................0.2g
Trace elements.............................................1.0mL
pH 4.0 ± 0.2 at 25°C
Trace Elements:
Composition per liter:
CaCl 2 ·2H 2 O...........................................0.66g
Na 2 MoO 4 ·2H 2 O .......................................0.3g
ZnSO 4 ·7H 2 O...........................................0.18g
CoCl 2 ·6H 2 O ..........................................0.18g
CuSO 4 ·5H 2 O...........................................0.16g
MnSO 4 ·4H 2 O...........................................0.15g
H 3 BO 3 .................................................0.1g
Preparation of Trace Elements: Add components to 1.0L of dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust medium to pH 4.0 with H 2 SO 4 . Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Bacillus cycloheptanicus.