Baird-Parker Agar, Supplemented

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baird-Parker Agar, Supplemented

培养基配方
Composition per liter:
Agar....................................................17.0g
Glycine.................................................12.0g
Sodium pyruvate.........................................10.0g
Pancreatic digest of casein.............................10.0g
Beef extract.............................................5.0g
LiCl.....................................................5.0g
Yeast extract............................................1.0g
RPF supplement........................................100.0mL
pH 7.0 ± 0.2 at 25°C
RPF Supplement:
Composition per 100.0mL:
Bovine fibrinogen...............................................3.75g
Trypsin inhibitor..............................................25.0mg
K2TeO3 ........................................................25.0mg
Rabbit plasma..................................................25.0mL
Caution: Potassium tellurite is toxic.
Preparation of RPF Supplement: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except RPF supple-
ment, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of
filter-sterilized RPF supplement. Mix thoroughly but gently. Pour into
sterile Petri dishes.
Use: For the selective isolation and enumeration of coagulase-positive
staphylococci from food, skin, soil, air, and other materials. For the dif-
ferentiation and identification of staphylococci on the basis of their
ability to coagulate plasma. Colonies surrounded by an opaque zone of
coagulated plasma are diagnostic for Staphylococcus aureus.