BAGG HiVeg Broth Base with Glycerol (Buffered Azide Glucose Glycerol HiVeg Broth Base)

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BAGG HiVeg Broth Base with Glycerol
(Buffered Azide Glucose Glycerol HiVeg Broth Base)

培养基配方
Composition per liter:
Plant hydrolysate No.1...........................................20.0g
Glucose ..........................................................5.0g
NaCl..............................................................5.0g
K2HPO4 ...........................................................4.0g
KH2PO4 ...........................................................1.5g
NaN3 .............................................................0.5g
Bromcresol Purple ..............................................0.015g
Glycerol ........................................................5.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium without glycerol is available as a premixed
powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium:  Add 5.0mL of glycerol to 900.0mL of
distilled/deionized water. Add remaining components and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distrib-
ute into tubes in 10.0mL volumes. Autoclave for 15 min at 10 psi
pressure–115°C.
Use: For the cultivation of fecal streptococci from a variety of clinical
and nonclinical specimens. It is recommended for qualitative presump-
tive and confirmatory tests for fecal streptococci.