BAGG Broth (Buffered Azide Glucose Glycerol Broth)

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BAGG Broth
(Buffered Azide Glucose Glycerol Broth)

培养基配方
Composition per liter:
Pancreatic digest of casein...............................10.0g
Peptic digest of animal tissue ...........................10.0g
Glucose....................................................5.0g
NaCl.......................................................5.0g
K2HPO4 ....................................................4.0g
KH2PO4 ....................................................1.5g
NaN3 ......................................................0.5g
Bromcresol Purple........................................0.015g
Glycerol .................................................5.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium:  Add 5.0mL of glycerol to 900.0mL of
distilled/deionized water. Add remaining components and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distrib-
ute into tubes in 10.0mL volumes. Autoclave for 15 min at 10 psi
pressure–116°C.
Use: For the cultivation of fecal streptococci from a variety of clinical
and nonclinical specimens. It is recommended for qualitative presump-
tive and confirmatory tests for fecal streptococci.