Arylsulfatase Agar (Wayne Sulfatase Agar)

[所属分类:培养基配方] [发布时间:2019-11-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Arylsulfatase Agar
(Wayne Sulfatase Agar)
Composition per liter:
Agar............................................................................................15.0g
Na2HPO4 .......................................................................................2.5g
L- Asparagine.................................................................................1.0g
KH2PO4 .........................................................................................1.0g
K2HPO4 .........................................................................................1.0g
Trisodium phenolphthalein sulfate .............................................0.65g
Pancreatic digest of casein............................................................0.5g
Ferric ammonium citrate.............................................................0.05g
MgSO4 ·7H2O..............................................................................0.01g
CaCl2 ·2H2O ...............................................................................0.5mg
ZnSO4 ·7H2O..............................................................................0.1mg
CuSO4 ........................................................................................0.1mg
Glycerol ...................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add glycerol to approximately 800.0mL
of distilled/deionized water. Mix thoroughly. Add remaining compo-
nents and bring volume to 1.0L with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool tubes in an upright
position.
Use: For the biochemical differentiation of species of Mycobacterium.
Inoculate tubes with Mycobacterium cultures and incubate aerobically
at 35°C for 3–14 days. Add 0.5–1.0mL of 2N Na 2 CO 3 to each tube and
observe color change within 30 min. Development of a pink color is
indicative of Mycobacterium fortuitum or Mycobacterium chelonae.
Mycobacterium tuberculosis gives a negative reaction.