Artificial Deep Lake Medium

[所属分类:培养基配方] [发布时间:2019-11-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Artificial Deep Lake Medium
Composition per liter:
NaCl..........................................................................................180.0g
MgCl2·6H2O ...............................................................................75.0g
Noble agar...................................................................................15.0g
Sodium succinate........................................................................10.0g
MgSO4 ·7H2O................................................................................7.4g
KCl................................................................................................7.4g
CaCl2 ·2H2O ..................................................................................1.0g
Yeast extract..................................................................................1.0g
Vitamin solution.......................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Vitamin Solution:
Composition per liter:
Biotin.......................................................................................30.0mg
Cyanocobalamin......................................................................20.0mg
Thiamine·HCl..........................................................................10.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize and add aseptically to sterile basal medium.
Preparation of Medium:  Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust medium to pH 7.4.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add 10.0mL of vitamin solution. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation and maintenance of Halobacterium lacuspro-
fundi.