Arginine Dihydrolase Broth

[所属分类:培养基配方] [发布时间:2019-11-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Arginine Dihydrolase Broth
Composition per liter:
L -Arginine...................................................................................10.0g
NaCl..............................................................................................5.0g
Agar..............................................................................................3.0g
Peptone .........................................................................................1.0g
K2HPO 4 .........................................................................................0.3g
Bromcresol Purple....................................................................0.016g
pH 6.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH so that it will be
6.5 ± 0.2 after sterilization. Distribute into 16 × 150mm screw-capped
tubes in 5.0mL volumes. Autoclave medium with loosely capped tubes for
10 min at 15 psi pressure–121°C. Screw the caps on tightly for storage and
after inoculation.
Use : For the cultivation and differentiation of bacteria based on their
ability to decarboxylate the amino acid arginine. Bacteria that decar-
boxylate arginine turn the medium turbid purple.