Arenavirus Plaquing Medium

[所属分类:培养基配方] [发布时间:2019-11-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Arenavirus Plaquing Medium
Composition per liter:
Eagle’s basal medium...................................................................1.0L
Agarose solution...........................................................................1.0L
Fetal calf serum, inactivated..................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Eagle’s Basal Medium:
Composition per liter:
HEPES (N-2-Hydroxyethylpiperazine-
N′-2-ethanesulfonic acid) buffer ..........................................9.53g
NaCl..............................................................................................6.8g
NaHCO3 ........................................................................................2.2g
Glucose .........................................................................................1.0g
KCl................................................................................................0.4g
CaCl2 ·2H2O...................................................................................0.2g
NaH2PO4 ...................................................................................0.125g
MgSO4 ·7H2O................................................................................0.1g
L- Isoleucine...............................................................................0.026g
L- Leucine...................................................................................0.026g
L- Lysine.....................................................................................0.026g
L- Threonine...............................................................................0.024g
L- Valine ...................................................................................0.0235g
L- Tyrosine..................................................................................0.018g
L- Arginine ...............................................................................0.0174g
L- Phenylalanine.......................................................................0.0165g
L- Cystine ...................................................................................0.012g
L- Histidine..................................................................................8.0mg
L- Methionine..............................................................................7.5mg
L- Tryptophan..............................................................................4.0mg
Inositol.......................................................................................1.8mg
Biotin .........................................................................................1.0mg
Calcium pantothenate ................................................................1.0mg
Choline chloride.........................................................................1.0mg
Folic acid....................................................................................1.0mg
Nicotinamide..............................................................................1.0mg
Pyridoxal·HCl............................................................................1.0mg
Thiamine·HCl ............................................................................1.0mg
Riboflavin ..................................................................................0.1mg
pH 7.2–7.4 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Eagle’s Basal Medium:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 7.0 with NaOH. Filter sterilize.
Agarose Solution:
Composition per liter:
Agarose.......................................................................................20.0g
Preparation of Agarose Solution: Add agarose to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium:  To 1.0L of sterile Eagle’s basal medium,
aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of
fetal calf serum. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of animal tissue culture cells used for the
growth of arenaviruses.