Arabinose Agar Base with Selective Supplement

[所属分类:培养基配方] [发布时间:2019-11-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Arabinose Agar Base with Selective Supplement
Composition per liter:
Peptone, special ..........................................................................23.0g
Agar............................................................................................15.0g
Arabinose....................................................................................10.0g
NaCl..............................................................................................5.0g
Corn starch....................................................................................1.0g
Phenol Red....................................................................................0.1g
Selective supplement solution.................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Thallium acetate............................................................................0.2g
Nalidixic acid...........................................................................25.0mg
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Adjust pH to 7.8. Gently heat and bring to
boiling. Distribute into tubes or flasks. Gently heat and bring to boil.
Do not autoclave. Cool to 50°C. Add selective supplement solution.
Mix thoroughly. Pour into sterile Petri dishes.
Use:  For selective isolation of Enterococcus faecium from feces, sew-
age, and water supplies.