​Aquaspirillum Medium (DSMZ Medium 888)

[所属分类:培养基配方] [发布时间:2019-11-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aquaspirillum Medium
(DSMZ Medium 888)
Composition per 1026mL:
Casamino acids.............................................................................1.5g
(NH4)2SO4 ....................................................................................1.0g
MgSO4·7H2O................................................................................1.0g
Agar..............................................................................................0.5g
CaCl2 ·6H2O .............................................................................30.0mg
Na2H2PO4 ................................................................................10.0mg
Sodium succinate solution.......................................................10.0mL
Thiosulfate solution.................................................................10.0mL
Standard vitamin solution..........................................................5.0mL
Trace elements solution SL-10..................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Standard Vitamin Solution:
Composition per 100.0mL:
Thiamine-HCl·2H 2 O................................................................50.0mg
Nicotinic acid...........................................................................50.0mg
Pyridoxine-HCl........................................................................50.0mg
Ca-pantothenate.......................................................................50.0mg
Riboflavin................................................................................10.0mg
Vitamin B 12 ..............................................................................1.0mg
Folic acid ...................................................................................0.2mg
Biotin.........................................................................................0.1mg
Preparation of Standard Vitamin Solution : Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O...................................................................................1.5g
H3BO3 ....................................................................................300.0mg
CoCl2 ·6H2O...........................................................................190.0mg
MnCl2 ·4H2O ..........................................................................100.0mg
ZnCl2 ........................................................................................70.0mg
Na2MoO4 ·2H2O.......................................................................36.0mg
NiCl2 ·6H2O..............................................................................24.0mg
CuCl2 ·2H2O...............................................................................2.0mg
HCl (25% solution)....................................................................7.7mL
Preparation of Trace Elements Solution SL-10: Add
FeCl2 ·4H 2 O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/
deionized water and bring volume to 1.0L. Add remaining compo-
nents. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at
15 psi pressure–121°C. Cool to room temperature.
Thiosulfate Solution:
Composition per 10.0mL:
Na2S2O3 ·5H2O..............................................................................1.0g
Preparation of Thiosulfate Solution:  Add Na2S2O3 ·5H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-
ature.
Sodium Succinate Solution:
Composition per 10.0mL:
Sodium succinate..........................................................................1.0g
Preparation of Sodium Succinate Solution:  Add sodium succi-
nate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature.
Preparation of Medium: Add components, except sodium succi-
nate solution, thiosulfate solution, standard vitamin solution, and trace
elements solution SL-10, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add 10.0mL sterile so-
dium succinate solution, 10.0mL sterile thiosulfate solution, 5.0mL
sterile standard vitamin solution, and 1.0mL sterile trace elements so-
lution SL-10. Mix thoroughly. Adjust pH to 7.5. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Aquaspirillum spp.