Aquaspirillum Heterotrophic Agar

[所属分类:培养基配方] [发布时间:2019-11-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aquaspirillum Heterotrophic Agar
Composition per liter:
Noble agar...................................................................................15.0g
Na2HPO4 ·12H2O...........................................................................9.0g
KH2PO4 .........................................................................................1.5g
NH4Cl ...........................................................................................1.0g
Sodium succinate..........................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
CaCl2 ·2H2O.................................................................................0.01g
Ferric ammonium citrate............................................................5.0mg
Trace elements solution .............................................................3.0mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
H3BO3 ......................................................................................30.0mg
CoCl2 ·6H2O .............................................................................20.0mg
ZnSO4 ·7H2O............................................................................10.0mg
MnCl2 ·4H2O...............................................................................3.0mg
Na2MoO4·2H2O .........................................................................3.0mg
NiCl2 ·6H2O................................................................................2.0mg
CuCl2 ·2H2O ...............................................................................1.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to double-distilled wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the heterotrophic cultivation and maintenance of Aquaspiril-
lum autotrophicum.