APT HiVeg Agar

[所属分类:培养基配方] [发布时间:2019-11-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

APT HiVeg Agar
Composition per liter:
Agar............................................................................................15.0g
Plant hydrolysate ........................................................................12.5g
Glucose.......................................................................................10.0g
Yeast extract..................................................................................7.5g
NaCl..............................................................................................5.0g
KH2PO4 .........................................................................................5.0g
Sodium citrate...............................................................................5.0g
Na2CO3 .......................................................................................1.25g
MgSO4 ·7H2O................................................................................0.8g
Polysorbate 80 ..............................................................................0.2g
MnCl2 ·4H2O ...............................................................................0.14g
FeSO4 ·7H2O................................................................................0.04g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and enumeration of bacteria, especially heter-
ofermentative lactobacilli, from meat and other foods. For the cultiva-
tion of streptococci.