APT Broth

[所属分类:培养基配方] [发布时间:2019-11-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

APT Broth
Composition per liter:
Pancreatic digest of casein..........................................................12.5g
Glucose .......................................................................................10.0g
Yeast extract..................................................................................7.5g
NaCl..............................................................................................5.0g
K2HPO4 .........................................................................................5.0g
Sodium citrate...............................................................................5.0g
Na2CO3 .......................................................................................1.25g
MgSO4·7H2O................................................................................0.8g
Polysorbate 80...............................................................................0.2g
MnCl2·4H2O................................................................................0.14g
FeSO4·7H2O................................................................................0.04g
Thiamine·HCl ............................................................................1.0mg
pH 7.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi—118°–121°C.
Use: For the cultivation of lactic acid bacteria. For the cultivation of
heterofermentative lactobacilli from meat and other foods. The Amer-
ican Public Health Association recommends adding 100.0μg/L of thi-
amine to this medium.