Apple Juice Yeast Extract Medium (AJYE Medium)

[所属分类:培养基配方] [发布时间:2019-11-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Apple Juice Yeast Extract Medium
(AJYE Medium)
Composition per 1200.0mL:
Agar ............................................................................................30.0g
Yeast extract................................................................................10.0g
Apple juice....................................................................................1.0L
pH 4.8 ± 0.2 at 25°C
Preparation of Medium: Add yeast extract to 1.0L of apple juice.
Mix thoroughly. Adjust pH to 4.8. Autoclave for 10 min at 9 psi pres-
sure–114°C. Cool to 45°–50°C. In a separate flask, add agar to
200.0mL of distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically combine the
sterile apple juice solution with the sterile agar solution. Mix thorough-
ly. Pour into sterile Petri dishes.
Use: For the cultivation of Zymomonas species.