Antimycin Medium

[所属分类:培养基配方] [发布时间:2019-11-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Composition per liter:
Yeast extract................................................................................20.0g
Peptone .......................................................................................10.0g
Glycerol ...................................................................................30.0mL
Antimycin solution ..................................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Antimycin Solution:
Composition per 10.0mL:
Antimycin..................................................................................1.0mg
Preparation of Antimycin Solution: Add antimycin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antimycin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 5.5 with
HCl. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add 10.0mL of sterile antimycin solution. Mix thoroughly.
Aseptically distribute into sterile tubes.
Use:  For the cultivation and maintenance of Candida utilis.