Antibiotic Medium 9 (Polymyxin Base Agar)

[所属分类:培养基配方] [发布时间:2019-10-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Medium 9
(Polymyxin Base Agar)
Composition per liter:
Agar ............................................................................................20.0g
Pancreatic digest of casein..........................................................17.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K2HPO4 .........................................................................................2.5g
Glucose .........................................................................................2.5g
pH 7.2 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For antibiotic assay testing. For base agar for the plate assay of
carbenicillin, colistimethate, and polymyxin B.