Antibiotic Medium 1 with Tetracycline, Streptomycin, and Chloramphenicol

[所属分类:培养基配方] [发布时间:2019-10-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Medium 1 with Tetracycline, Streptomycin,
and Chloramphenicol
Composition per liter:
Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................6.0g
Pancreatic digest of casein............................................................4.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................1.5g
Glucose.........................................................................................1.0g
Antibiotic solution...................................................................10.0mL
pH 6.6 ± 0.1 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Tetracycline.................................................................................0.02g
Streptomycin...............................................................................0.02g
Chloramphenicol.........................................................................0.02g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the selective cultivation and maintenance of Salmonella
choleraesuis.