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YPDP Medium with 5′-TMP

[所属分类:培养基配方] [发布时间:2021-12-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Peptone .............................................................20.0g

Agar ..................................................................15.0g

Yeast extract......................................................10.0g

KH2PO4...............................................................1.5g

Thymidine-5′-monophosphate solution........ 10.0mL

Thymidine-5′-Monophosphate Solution:

Composition per 10mL:

Thymidine-5′-monophosphate ....................100.0mg

Preparation of Thymidine-5′-Monophos

phate Solution: Add thymidine-5′-monophos

phate to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept thymidine-5′-monophosphate solution, to dis

tilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C. Aseptically add 10.0mL of sterile thymi

dine-5′-monophosphate solution. Mix thoroughly.

Pour into sterile Petri dishes or aseptically distribute

into sterile tubes.

Use: For the cultivation and maintenance of Saccharo

myces cerevisiae.