Antibiotic HiVeg Assay Medium No. 32

[所属分类:培养基配方] [发布时间:2019-10-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic HiVeg Assay Medium No. 32
Composition per liter:
Agar ............................................................................................15.0g
Plant peptone.................................................................................6.0g
Plant hydrolysate...........................................................................4.0g
Yeast extract..................................................................................3.0g
Plant extract ..................................................................................1.5g
Glucose .........................................................................................1.0g
MnSO4 ·4H2O................................................................................0.3g
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For preparing inoculum of Bacillus subtilis ATCC 6633 during
assay of dihydrostreptomycin and vancomycin.