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Yeast Synthetic Minimal Medium

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]
Yeast Synthetic Minimal Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

D-Glucose..........................................................20.0g
Agar ..................................................................15.0g
(NH4)2SO4...........................................................5.0g
KH2PO4...............................................................1.0g
MgSO4·7H2O ......................................................0.5g
NaCl....................................................................0.1g
CaCl2·2H2O.........................................................0.1g
Inositol .............................................................2.0mg
H3BO3 ..............................................................0.5mg
ZnSO4·7H2O ....................................................0.4mg
MnSO4·4H2O ...................................................0.4mg
Thiamine·HCl ..................................................0.4mg
Pyridoxine·HCl ................................................0.4mg
Niacin...............................................................0.4mg
Calcium pantothenate ......................................0.4mg
p-Aminobenzoic acid.......................................0.2mg
Riboflavin ........................................................0.2mg
FeCl3 ................................................................0.2mg
Na2MoO4·4H2O ...............................................0.2mg
KI .....................................................................0.1mg
CuSO4·5H2O ..................................................0.04mg
Folic acid.......................................................... 2.0μg
Biotin ............................................................... 2.0μg
pH 5.6 ± 0.2 at 25°C
Preparation of Medium: Add agar to 900.0mL
of distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. In a separate flask, add re
maining components to 100.0mL of distilled/deion
ized water. Mix thoroughly. Filter sterilize.
Aseptically combine the two sterile solutions. Mix
thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of a wide variety of het
erotrophic microorganisms.
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