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Yeast Extract Peptone Starch Agar

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................18.0g

Soluble starch....................................................10.0g

Peptone .............................................................10.0g

CaCO3 .................................................................5.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................3.0g

KH2PO4...............................................................0.5g

K2HPO4...............................................................0.5g

MgSO4·7H2O......................................................0.3g

Sodium citrate.................................................0.027g

NaCl..................................................................0.01g

MnSO4·5H2O....................................................0.01g

CuSO4·5H2O....................................................1.0mg

CoCl2·6H2O .....................................................1.0mg

FeSO4·7H2O.....................................................1.0mg

Preparation of Medium: Add components to tap

water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus

species that utilize starch as a carbon source.