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Yeast Carbon Base, 10X (Wickerham Carbon Base Broth)

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Carbon Base, 10X

(Wickerham Carbon Base Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................10.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O ........................................................0.1g

DL-Methionine...................................................0.02g

DL-Tryptophan...................................................0.02g

L-Histidine·HCl .................................................0.01g

Inositol .............................................................2.0mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyridoxine........................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

KI .....................................................................0.1mg

CuSO4·5H2O..................................................0.04mg

Folic Acid .........................................................2.0μg

Biotin ................................................................2.0μg

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Filter sterilize.

Use: Used as a base to which different nitrogen sources

may be added. For the cultivation and differentiation of

bacteria based on their ability to utilize diverse added ni

trogen sources.