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Vibrio parahaemolyticus Sucrose Agar (VPSA) (BAM M191)

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Vibrio parahaemolyticus Sucrose Agar

(VPSA)

(BAM M191)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................30.0g

Agar ..................................................................15.0g

Sucrose..............................................................10.0g

Yeast extract........................................................7.0g

Tryptose ..............................................................5.0g

Pancreatic digest of casein..................................5.0g

Bile salts No. 3....................................................1.5g

Bromthymol Blue ...........................................0.025g

pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not autoclave. Cool to 50°C. Pour into sterile Petri

dishes in 20.0mL volumes. Allow plates to dry be

fore using.

Use: For the isolation, cultivation, and differentia

tion of Vibrio parahaemolyticus from seafood. Vibrio

parahaemolyticus and Vibrio vulnificus appear as

blue to green colonies. Other Vibrio species appear as

yellow colonies.