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Uric Acid Agar
[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]
Uric Acid Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ..................................................................15.0g
Na2HPO4·12H2O ................................................9.0g
NaCl....................................................................5.0g
KH2PO4 ..............................................................1.5g
Meat extract ........................................................1.0g
Yeast extract........................................................1.0g
MgSO4·7H2O......................................................0.2g
MnCl2·4H2O ..................................................20.0mg
CaCl2................................................................1.2mg
Glucose-uric acid solution .......................... 100.0mL
Glucose-Uric Acid Solution:
Composition per 100.0mL:
Glucose ...............................................................5.0g
Uric acid..............................................................0.4g
Preparation of Glucose-Uric Acid Solution:
Add components to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Filter
sterilize. Warm to 50°C.
Preparation of Medium: Add components, ex
cept glucose-uric acid solution, to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile glucose-uric acid
solution. Mix thoroughly. Pour into sterile Petri dish
es or distribute into sterile tubes.
Use: For the cultivation and maintenance of Bacillus
species.