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培养基配方
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Urea Agar
[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]
Urea Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ..................................................................15.0g
Urea...................................................................10.0g
Na2HPO4·12H2O ................................................9.0g
NaCl....................................................................5.0g
KH2PO4 ..............................................................1.5g
Meat extract ........................................................1.0g
Yeast extract........................................................1.0g
MgSO4·7H2O......................................................0.2g
MnCl2·4H2O..................................................20.0mg
CaCl2 ...............................................................1.2mg
Glucose solution ......................................... 100.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose ...............................................................5.0g
Preparation of Glucose Solution: Add glucose
to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize. Warm to
50°C.
Preparation of Medium: Add components, ex
cept glucose solution, to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptical
ly add 100.0mL of sterile glucose solution. Mix thor
oughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and maintenance of Coryne
bacterium glutamicum.