Antibiotic Assay Medium No. 40

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium No. 40
Composition per liter:
Yeast extract................................................................................20.0g
Agar ............................................................................................10.0g
Glucose .......................................................................................10.0g
Casein enzymatic hydrolysate ......................................................2.5g
Peptone..........................................................................................2.5g
KH2 PO 4 .........................................................................................2.0g
Tween™ 80...................................................................................0.1g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the microbiological assay of Thiostrepton using Streptococ-
cus faecium ATCC 10541.