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Polymyxin Pyruvate Egg Yolk Mannitol Bromthymol Blue Agar (PEMBA)

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Polymyxin Pyruvate Egg Yolk Mannitol

Bromthymol Blue Agar

(PEMBA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 110.0mL:

Agar ....................................................................1.8g

D-Mannitol ..........................................................1.0g

Sodium pyruvate.................................................1.0g

Na2HPO4 ...........................................................0.25g

NaCl....................................................................0.2g

Peptone................................................................0.1g

KH2PO4...........................................................0.025g

Bromthymol Blue .............................................0.01g

MgSO4·7H2O ....................................................0.01g

Antibiotic inhibitor ......................................... 5.0mL

Egg yolk emulsion (20% solution) ................. 5.0mL

pH 7.4 ± 0.2 at 25°C

Antibiotic Inhibitor:

Composition per 5.0mL:

Polymyxin B ................................................10,000U

Preparation of Antibiotic Inhibitor: Add com

ponents to distilled/deionized water and bring vol

ume to 5.0mL. Mix thoroughly. Filter sterilize.

Egg Yolk Emulsion, 20% Solution:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

NaCl (0.9% solution) .................................... 80.0mL

Preparation of Egg Yolk Emulsion, 20% So

lution: Soak eggs with 1:100 dilution of saturated

mercuric chloride solution for 1 min. Crack eggs and

separate yolks from whites. Mix egg yolks with 1

chicken egg. Measure 20.0mL of egg yolk emulsion

and add to 80.0mL of 0.9% NaCl solution. Mix thor

oughly. Filter sterilize. Warm to 45°–50°C.

Preparation of Medium: Add components, ex

cept antibiotic inhibitor and egg yolk emulsion, 20%,

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile an

tibiotic inhibitor and egg yolk emulsion, 20%. Mix

thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation of Bacillus cereus.

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Polymyxin Pyruvate Egg Yolk Mannitol Bromthymol Blue Agar (PEMBA)