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PM Indicator Agar

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

PM Indicator Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................5.25g

Peptone................................................................5.0g

Beef extract.........................................................3.0g

Pancreatic digest of casein..................................1.7g

Sorbitan monooleate complex ............................1.0g

NaCl....................................................................0.5g

Papaic digest of soybean meal............................0.3g

K2HPO4.............................................................0.25g

Bromcresol Purple ............................................0.06g

pH 7.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not autoclave. Inoculate medium with a suspension

of Bacillus stearothermophilus spores. Pour into

100mm flat-bottomed Petri dishes in 6.0mL vol

umes. Use medium immediately.

Use: For the rapid detection of trace amounts of pen

icillin in milk using the AOAC Bacillus stearother

mophilus Qualitative Disc Method II.