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Pisu Medium

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Pisu Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1512.0mL:

Agar base .................................................... 960.0mL

Horse serum, sterile .................................... 360.0mL

L-Cystine solution....................................... 180.0mL

Lead acetate solution .................................... 12.0mL

pH 6.8 ± 0.2 at 25°C

Agar Base:

Composition per liter:

Proteose peptone No. 3.....................................20.0g

Agar ....................................................................7.0g

NaCl....................................................................5.0g

Meat extract ........................................................4.0g

Preparation for Agar Base: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Dissolve in steam for 15 min at 0 psi

pressure–100°C. Cool to 45°–50°C. Adjust pH to

7.5. Filter sterilize. Distribute into 200.0mL Erlen

meyer flasks in 80.0mL volumes. Autoclave for 60

min at 0 psi pressure–100°C. Cool to 60°C.

L-Cystine Solution:

Composition per 200.0mL:

L-Cystine.............................................................2.0g

Preparation of L-Cystine Solution: Add L-cys

tine to distilled/deionized water and bring volume to

200.0mL. Mix thoroughly. Filter sterilize.

Lead Acetate Solution:

Composition per 100.0mL:

Lead acetate ......................................................10.0g

Preparation of Lead Acetate Solution: Add

lead acetate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation for Medium: To each flask contain

ing 80.0mL of cooled, sterile agar base, aseptically

add 30.0mL of sterile horse serum, 15.0mL of sterile

L-cystine solution, and 1.0mL of sterile lead acetate

solution. Mix thoroughly. Aseptically distribute into

small sterile tubes in 2.0–3.0mL volumes.

Use: For the cultivation and differentiation of bacte

ria based on their ability to produce cystinase. Cysti

nase-producing bacteria turn the medium black.