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Phenol Red Glucose Broth with NaCl (BAM M122)

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Glucose Broth with NaCl

(BAM M122)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................30.0g

Proteose peptone No. 3.....................................10.0g

Glucose ...............................................................5.0g

Beef extract.........................................................1.0g

Phenol Red solution........................................ 7.2mL

pH 7.4 ± 0.2 at 25°C

Source: This medium without added NaCl is avail

able as a premixed powder from BD Diagnostics.

Phenol Red Solution:

Composition per 10.0mL:

Phenol Red......................................................0.025g

Preparation of Phenol Red Solution: Add Phe

nol Red to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 7.4 if necessary. Dis

tribute into tubes containing an inverted Durham

tube. Fill each tube with 10.0mL of medium. Auto

clave for 10 min at 13 psi pressure–118°C.

Use: For determination of the ability of halophilic

Vibrio spp. to ferment glucose. Fermentation is deter

mined by the production of acid—broth turns yel

low—and formation of gas—bubble trapped in

Durham tube.